Discourse of post-human subject is formed by the critical reflection on the discourse of modernity subject. In this regard, the“ cyborg” discourse of subject, informationalism discourse of subject and“ universal-vitality”discourse of subject can be seen as the representative forms of discourse of post-human subject in Western countries. When it comes to the reflection on solipsism, autonomy and modernity subject of possession, discourse of post-human subject has made some achievements. However, there are still some limitations the subject concept of adherent entity, the relationship mode between the subject and the object, and the residual anthropocentrism By putting forward the “mediated subjectivity” discourse, this paper aims to provide a new explanation for post-human subject. As mediated subjectivity discourse takes the mediated“ cyborg” life forms as the material basis, the basic structure of the mediated“ Cyborg” refers to “individual(body-consciousness)- media-identity”. Specially presented as actively connecting, inviting, gathering, tolerating , and interacting with the outside world,the mediated subjectivity also practically realizes the communication between the subject,the world, as well as the existing forms of post-human subject which are intertwined and infused with one another.